|Jessica Hekman meets friendly fox.|
Hi Mia and Julie,
One of the things I love most about dogs is how friendly they are. What's the biggest difference between a dog and a wolf? The dog probably wants to come say hi to you. The wolf is scared of you, and may demonstrate that fear through aggression if you get too close.
But not ALL dogs are friendly, right? If “friendliness” versus “fear” was on a spectrum, most dogs would be on the “friendly” end, but some would be down towards the “fear” end with the wolves. This is what I study: what is going on in the brain of fearful dogs to make them scared of things? What are the mechanisms that are different? I'm interested in the wiring in the brain, hormone differences, neurotransmitter differences, and more.
Right now, the way I'm studying fear in dogs is by studying fear in foxes. I know you know about the famous population of tame foxes at the Institute of Cytology and Genetics in Novosibirsk, Russia. I got to go meet these foxes recently!
They have been bred for tameness for more than forty generations, and are a lot like dogs in their friendliness and lack of fear. In the lab where I work, we compare these foxes to another group of foxes that have been selected for fearfulness/aggression. Through comparing these very different groups, we to try to understand the biological mechanisms behind their personality differences. Foxes are evolutionarily close to dogs, and because these foxes have carefully controlled genetics and environments, they are easier to study than pet dogs are. (I still hope to transition to working directly with pet dogs some day, though.)
|Hekman with friendly fox.|
We are still very much at the stage of just trying to figure out where in the enormous genome (3.3 billion nucleotides!) the personality differences between tame and aggressive foxes come from. (By the way, various efforts looking at personality differences in humans are at the same stage.)
To answer this question, we looked for differences not just between individual foxes, but between the group of 20 tame foxes and the group of 20 aggressive foxes. With 8,437 SNPs you had better bet we used computers for this. It was a surprise to me when I got into modern genomics just how much of the work deals with complex computer algorithms to process the massive amounts of data we're dealing with!
|Hekman with fox at the Institute of Cytology and Genetics.|
Imagine that the tame foxes all had, “The quick brown fox jumps over the lazy dog” in their version of chapter two, but the aggressive foxes all had, “The quick brown fox jumps over the lazy doc” in their version. Basically, by finding places where the tame foxes all had one version, and the aggressive foxes all had a different version, we were finding places in the genome where we hoped to find important differences, changes that help cause the tame fox personality phenotype. We found 28 regions like this, but focused on three of them as the most interesting.
Next we looked at the genes that these changes might affect, because finding gene differences was the point of the whole exercise. Remember, we still don't know most of what most of the genes out there do! This is really dark side of the moon stuff, and everyone is still guessing about what's going on in the genome, human or fox. But here are some interesting genes we found. I'm including a “wild hypothesis” with some of them. These hypotheses are probably wrong, but I hope they help to show why these genes are so interesting to us...
GRIN2B: this gene codes for a receptor for one of the major neurotransmitters in the brain, glutamate. Glutamate is involved in learning and memory. Wild hypothesis: maybe tame foxes are less afraid because of a difference in how they learn about what to fear.
GABARAPL1 (GEC1): this gene is involved with opioids, molecules in the brain that help us feel good. Wild hypothesis: maybe tame foxes are more friendly because social interactions feel different (better) to them.
COUP-TFII (NR2F2): this gene is important during embryonic brain development, especially in the amygdala, a part of the brain that tells us when to be afraid. This gene also influences expression of oxytocin, a neurotransmitter which functions in social bonding.
These genes are extremely interesting, but even more than that, this work helped our lab implicate specific regions of the genome in the differences between tame and aggressive foxes. That list of regions will prove invaluable as we do more work in the future, using different tools to examine the tame fox genome and seeing which tools point at the same regions.
Tame fox kisses to you both!
Dr. Jessica Perry Hekman graduated Tufts Cummings School of Veterinary Medicine in 2012, where in addition to her DVM she received an MS for work on stress behavior and cortisol levels in hospitalized dogs. She completed a shelter medicine internship at the University of Florida in 2013, and is now a PhD student at the University of Illinois at Urbana-Champaign, where she works at Kukekova Lab. Her research interests are in the biological mechanisms behind fearfulness in dogs. You can follow her at @dogzombieblog.
Images copyright Jessica Hekman.
 Trut, Lyudmila N., I. Z. Plyusnina, and I. N. Oskina. "An experiment on fox domestication and debatable issues of evolution of the dog." Russian Journal of Genetics 40.6 (2004): 644-655.
 Huang, Shihhui, et al. "Selection for tameness, a key behavioral trait of domestication, increases adult hippocampal neurogenesis in foxes." Hippocampus (March 2015).
 Johnson, Jennifer L. et al. "Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes)." PLoSONE (2015).
© 2015 Jessica Perry Hekman | Do You Believe in Dog?